Skip to main content
. Author manuscript; available in PMC: 2015 Oct 15.
Published in final edited form as: J Virol Methods. 2013 Sep 18;195:18–25. doi: 10.1016/j.jviromet.2013.09.003

Table 2.

Primers and probes used for real-time RT-PCR amplification of A/Victoria/361/2011 (H3N2).

Primers/probesa Gene Nucleotides Sequence 5′→3′
NSVicF149 NS 149–169 TCGGTCTAGACATCAAAGCAG
NSVicR234 NS 211–234 TTTAAGTGCCTCATCAGATTCTTC
NSVicP170 NS 170–193 FAM-CCACCCATGTTGGAAAGCAAATTG-BHQ1
MVicF809 M 809–833 TGGATTCTTGATCGTCTTTTTTTCA
MVicR875 M 854–874 GCCTCTTTTAAGGCCGTGTTT
MVicP834 M 835–851 FAM-ATGCGTCTATCGACTCT-BHQ1
NAVicF1106 NA 1106–1125 CGTCACGCTTAGGGTATGAA
NAVicR1215 NA 1197–1216 AACCGGACCTATCACCTCTG
NAVicP1136 NA 1136–1158 FAM-TCATTGAAGGCTGGTCCAACCCT-BHQ1
NPVicF838 NP 838–858 GCGTATGGACCTGCAGTATCC
NPVicR908 NP 888–908 GGGTCTATTCCCACCAAGGAA
NPVicP861 NP 861–878 FAM-TGGGTACGACTTCGAAAA-BHQ1
PAVicF1280 PA 1280–1299 AAATTGGAGAGGACGTAGCC
PAVicR1360 PA 1341–1360 TACAATGGGACACCTCTGCT
PAVicP1300 PA 1300–1323 FAM-CCAATTGAGCACATTGCAAGCATG-BHQ1
PB1VicF659 PB1 659–679 GAGCTTTGACATTGAACACGA
PB1VicR731 PB1 712–731 GGTGTTGCAATAGCCCTTCT
PB1VicP704r PB1 680–704 FAM-TTGCCTCTCTCTGCATCTTTGGTCA-BHQ1
PB2VicF999 PB2 999–1017 AAGCGGGTCATCAGTCAAA
PB2VicR1076 PB2 1052–1076 CCCTCATGTACTCTTATTCTCAATG
PB2-VicP1050r PB2 1024–1050 FAM-TTGGAGATTGCCTGTAAGCACCTCTTC-BHQ1
a

F and R in the primer name indicate forward and reverse direction, respectively. P indicates probe, and r indicates probe in reverse direction.