Table 3.
Primers and probes used for real-time RT-PCR amplification of B/Texas/06/2011.
| Primers/probesa | Gene | Nucleotides | Sequence 5′ →3′ |
|---|---|---|---|
| HATex_F226 | HA | 226–245 | ACAGATCTGGATGTGGCCTT |
| HATex_R287 | HA | 267–287 | GCTTTAGCAGAAGGTGTGGTC |
| HATex_P266r | HA | 247–266 | FAM-CCCACACACATTGGCCTGCC-BHQ1 |
| NATex_F718 | NA | 718–737 | ATAACTGATGGCCCAGCTTC |
| NATex_R837 | NA | 817–837 | GCATGTGCATTCCTCAGTATG |
| NATex_P779r | NA | 753–779 | FAM-CGGCCTTCTCGAATCTTAAGGAATCTG-BHQ1 |
| MTex_F256 | M | 256–275 | ATGGGAACAACAGCAACAAA |
| MTex_R358 | M | 339–358 | CATGGCCTTCTGCTATTTCA |
| MTex_P304r | M | 282–304 | FAM-TTCTCTCAGCCAGAATCAGGCCC-BHQ1 |
| NSTex_F377 | NS | 377–396 | GGAGGTGCCTTGATGACATA |
| NSTex_R461 | NS | 438–461 | TCTTTGTTGTTCATGTCCCTTAAT |
| NSTex_P426r | NS | 403–426 | FAM-TGGGCCATCAACATCATCTGGTTC-BHQ1 |
| NPTex_F159 | NP | 159–179 | AGCAACCACAAGCAGTGAAGA |
| NPTex_R298 | NP | 279–298 | TGAGTCCAGCTTTGACCATC |
| NPTex_P263r | NP | 243–263 | FAM-TCGCCCAGTTTCACCACCATG-BHQ1 |
| PATex_F260 | PA | 260–279 | TAGCATGGATGGTCCAAAGA |
| PATex_R338 | PA | 316–338 | TAATCAAACAAATCAGCCAGATA |
| PATex_P314r | PA | 288–314 | FAM-TTGGGAGTCTCTATCCCATGCTCTTGA-BHQ1 |
| PB1Tex_F141 | PB1 | 141–166 | TGAGTACTCGAACAAAGGAAAACAGT |
| PB1Tex_R215 | PB1 | 194–215 | GGCCCATTTGTTGGATCTATCA |
| PB1TexP168 | PB1 | 168–187 | FAM-TGTTTCTGACATCACAGGAT-BHQ1 |
| PB2Tex_F587 | PB2 | 587–607 | AAGGAACGATGATAACTCCCA |
| PB2Tex_R657 | PB2 | 637–657 | GAACCTTCTCCTGGCAACTAA |
| PB2Tex_P636r | PB2 | 614–636 | FAM-TTCCCTCTCGAGCATGTATGCCA-BHQ1 |
a
F and R in the primer name indicate forward and reverse direction, respectively. P indicates probe, and r indicates probe in reverse direction.