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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Int J Biol Macromol. 2015 Jan 17;75:37–43. doi: 10.1016/j.ijbiomac.2014.12.047

TABLE 2.

Growth of mutant E. coli strains in presence of melittin-amide and MRP-amide

Mutationa Growthb
On succinate plates
(Melittin-amide/MRP-amide)		 Growth yield in
limiting glucosec
(Melittin-amide/MRP-amide)
(%)
Wild-type ±/± 20/25
Null −/− 43/46
βY331W ±/± 22/24
βD380A +++/+++ 89/92
βD380Q +++/+++ 90/88
βD380R +++/+++ 82/83
βD386A +++/+++ 79/77
βD386Q +++/+++ 74/72
βD386R +++/+++ 70/69
βD380A/βD386A +++/+++ 75/70
βD380Q/βD386Q +++/+++ 72/75
βD380R/βD386R ++/++ 66/70
a

Wild-type, pBWU13.4/DK8; Null, pUC118/DK8. Data are means of four to six experiments each.

b

Growth on succinate plates after 3 days estimated by eye. ++++, normal growth; +++, less growth; ++, lesser growth; ±, insignificant growth; −, no growth.

c

Growth on limiting glucose is based on the growth of wild type in absence of peptides and used as 100%. Each individual experimental point is itself the mean of duplicate assay tubes. Data are derived from two-three separate membrane preparations. Results from separate membrane preparations were in excellent agreement.