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. Author manuscript; available in PMC: 2015 Oct 15.
Published in final edited form as: J Am Chem Soc. 2015 Feb 2;137(5):1738–1741. doi: 10.1021/jacs.5b00056

Figure 4.

Figure 4

Binding of quinazolinone 2 to PBP2a allosteric site, determined using a previously described procedure.17 (A) The change in the maximum fluorescence intensity gave a Kd of 6.9 ± 2 μg/mL (average of three experiments; nonlinear regression for data fitting with an R2 of 0.9997). (B) Emission scans of PBP2a intrinsic fluorescence with excitation at 280 nm. Antibiotic 2 was titrated in to give the final concentrations shown.