Figure 1. Analysis of FTDs.

(A) Schematic representation of the experimental strategy. (B) Approximately 3 × 10⁴ to 5 × 10⁴ cells from FTDs were incubated for 3 to 4 days in lymphocyte medium and then stained with panels of mutated epitope MHC tetramers. The cells were analyzed by flow cytometry (gated on the lymphocyte population). For each patient, the frequency of tetramer-positive cells is indicated (y axis) for each peptide screened (x axis). Patients 3713, 3466, and 3879 were screened for binding to HLA-A*02:01 tetramers and patient 3919 for binding to HLA-A1 tetramers. (C) Tetramer (Tet) staining for selected mutated epitopes identified for each patient. The percentage of positive cells is indicated. (D) Cultured FTD cells were incubated with T2 cells pulsed with the predicted mutated epitopes or incubated with T2 cells pulsed with irrelevant peptide (Ctrl, see Methods) for 16 hours, and IFN-γ secretion was measured by ELISA. Pt., patient.