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. 2015 Sep 21;125(10):3981–3991. doi: 10.1172/JCI82416

Figure 2. Sorting and expansion of mutated antigen–specific T cells.

Figure 2

(A) Tetramer-positive T cells were sorted from FTDs and expanded in vitro by stimulation with OKT3. Ten days later, T cells were assessed for tetramer binding. (B) T cells that were sorted and expanded in vitro were cocultured with T2 cells pulsed with the cognate mutated epitope or cocultured with T2 cells pulsed with the corresponding WT peptide. Sixteen hours after the start of the coculture, IFN-γ secretion was measured by ELISA. Results are presented as the mean ± SEM (n = 3). P < 0.05 by Student’s t test for the difference in IFN-γ secretion between mutated (Mut pep) and WT peptides (WT pep).