Skip to main content
. 2015 Sep 8;125(10):3915–3927. doi: 10.1172/JCI81086

Figure 3. Mars1Cy-tagged AVPR2 is responsive to desmopressin-stimulated (DDAVP) endocytosis and demonstrates the feasibility of 2-channel separation of external and internal protein pools using impermeant and membrane-permeant fluorogens that emit in the far-red.

Figure 3

(A) HEK293 cells expressing Mars1Cy-AVPR2 were incubated at 37°C with the indicated membrane-impermeant fluorogens for 5 minutes prior to stimulation with DDAVP. Images were acquired immediately upon agonist application and at 20 minutes after addition. (B) Sequential addition of impermeant fluorogen (either SCi1 or MG-2p, 10 minutes) followed by permeant fluorogen MG-ester (MGE) or SC1, respectively, for 30 minutes. Each panel is representative of 3 replicates. Scale bars: 16 μm. Magnification is constant across all time points.