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. 2015 Aug 31;125(10):3795–3808. doi: 10.1172/JCI81979

Figure 4. ZIC2 initiates OCT4 activation.

Figure 4

(A) ZIC2 deficiency reduces OCT4 expression. The indicated core stemness factors were analyzed in ZIC2 KO or control Hep3B cells. (B) OCT4 expression was detected by immunoblotting in ZIC2-deleted cells. (C) The expression levels of OCT4 downstream genes were detected in ZIC2 KO and control cells. (D) ZIC2 knockdown in HCC primary cells reduces OCT4 expression. (E) ZIC2 overexpression increases OCT4 expression. ZIC2 was overexpressed in HCC primary cells followed by OCT4 detection. (F) Correlation analysis of OCT4 and ZIC2 were analyzed based on their expression levels in HCC samples by quantitative RT-PCR. Z2KO, ZIC2 KO cell; oeVec, overexpressed vector; oeZIC2, overexpressed ZIC2. Data are shown as means ± SD. Two-tailed Student’s t test was used for statistical analysis. *P < 0.05; **P < 0.01; and ***P < 0.001. Data are representative of at least 3 independent experiments.