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. 2015 Sep 8;125(10):3809–3818. doi: 10.1172/JCI77095

Figure 4. Loss of Hepatic Trib1 leads to increased C/EBPa and C/EBPb protein levels, as well as decreased transcript levels.

Figure 4

(A) Western blot analysis of C/EBPα, C/EBPβ, and β-actin levels in livers of Trib1_fl/fl and Trib1_LSKO mice. Whole-liver protein extracts were made in PBS + protease inhibitors, and proteins were size-separated by SDS-PAGE. (B) Real-time RT-PCR analysis of Cebpa and Cebpb hepatic transcript levels in Trib_fl/fl and Trib1_LSKO mice (n = 5) 4 weeks after injection. TaqMan Realtime RT-PCR was performed using commercially available gene-expression probes on cDNA made from 1 μg total liver RNA. Results in B were analyzed by Student’s t test (**P ≤ 0.01).