Figure 5. Overexpression of mCebpa in livers of mice phenocopies the increased hepatic TG synthesis observed in Trib1_LSKO mice.

(A) Liver TG content in mice receiving 1 × 10¹² AAV_Null or AAV_mCebpa 2 weeks after injection after overnight fast (n = 5). (B) Hepatic lipogenic gene expression in AAV_Null and AAV_mCebpa mice 2 weeks after injection, as measured by TaqMan real-time RT-PCR from 1 μg total RNA. Values are relative expression compared with GAPDH housekeeping gene. (C) De novo lipogenesis in mice from A after overnight fast, refeeding, and fatty acid labeling with [3H]-Acetate. (D) Plasma TC, non-HDL cholesterol, and TG were measured after a 4-hour fast in mice (n = 6) 10 weeks after AAV_Null or AAV_mCebpa injection. Significance was determined in all panels by Student’s t test (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001).