Figure 6. C/EBPα is required for increased lipogenesis in Trib1_LSKO mice.

(A) Hepatic transcript levels of Cebpa in WT mice, Trib1_LSKO mice, or Trib1/Cebpa_dLSKO mice (n = 6) 2 weeks after injection with 1.5 × 10¹¹ gc of AAV-TBG-Cre. (B) Transcription of lipogenic genes in all 3 experimental groups. Gene expression values are relative to the Gapd housekeeping gene, with the WT group set to 1. (C) Production of fatty acids, diacylglycerol, and TG is shown in WT, Trib1_LSKO, and Trib1/Cebpa_dLSKO mice after injection with [3H]-acetate. Significance was determined in all panels by ANOVA followed by Tukey’s post-hoc test (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001).