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. 2015 Sep 14;125(10):3819–3830. doi: 10.1172/JCI79048

Figure 5. Administration of perhexiline increased HDL-C levels in vivo.

Figure 5

C57BL/6J mice placed on a HFD for 12 weeks were treated with DMSO or perhexiline maleate salt (10 mg/kg/d) for 5 consecutive days by gavage administration, and plasma samples were collected at day 7 (n = 10 per group). HDL-C (A), TC (B), LDL-C (C), and TG (D) levels were measured. *P < 0.05, Student’s t test. (E) The ABCA1-mediated cholesterol efflux capacity of serum from DMSO- or perhexiline-treated mice is expressed as the percentage of cholesterol efflux of total cell cholesterol (n = 10 per group). *P < 0.05, Student’s t test. Pooled serum samples from DMSO- or perhexiline-treated mice were assayed by HPLC, and cholesterol (F) and TG (G) levels (fractions 1 to 32) were determined. (H–K) KLF14-LKO and littermate control mice were treated with DMSO or perhexiline maleate salt (10 mg/Kg/d) for 5 consecutive days by gavage administration, and plasma samples were collected at day 7 (n = 5–8 for each genotype). HDL-C levels were determined (H) and ApoA-I levels were quantified by Western blot analysis (I) (n = 5–8 for each genotype). (J and K) qRT-PCR analysis showing the expression levels of Klf14 and Apoa1 in indicated groups. Data are expressed relative to 18S RNA (n = 5–8 for each genotype). Values represent mean ± SEM. *P < 0.05; **P < 0.01, 2-way ANOVA and multiple comparisons.