Fig. 5.

SNP calling based on DISR. a Nicotiana tabacum L. has 24 nuclear chromosomes, each of which contains multiple EcoRI cut sites (red marks). The genomic DNA is digested, bar coded with a population-specific sequence, and amplified resulting in multiple sequence reads from each of the RAD tag sites in the genome. Each sequence consists of a population-specific 5-bp barcode (black), the enzyme-recognition sequence (red), and the downstream sequence. b The de novo RAD tag pipeline compares all the sequenced reads and builds clusters of exactly matching tags. c Pair wise comparisons are made between all clusters. d There is a cluster in the locus that is SNP. e The consensus sequence for that RAD tag site within the population