Fig 3. MEF35 interacts with the MORF1, MORF2 and MORF8 proteins.

Yeast 2-hybrid (Y2H) assays reveal interactions with the mitochondrially located MORF1, the plastid MORF2 and the dual targeted MORF8. In a mutant of MORF1, the MEF35 target sites are not affected [22], some slight effects are seen upon knock-down in other assays [28]. In a respective MORF8 (also termed RIP1) mutant, editing at two of the three MEF35 target sites is reduced, suggesting that the interactions with MORF8 maybe functionally relevant. Controls include cotransfection of the pGBKT7-MEF35 construct with the pGADT7 vector without any MORF insert (empty) as well as the positive interaction control of murine p53 (pGBKT7-53) with the SV40 large T-antigen in pGADT7 (T) and no connection of human lamin C (pGBKT7-Lam) with the SV40 large T-antigen (T). The fusion protein pGBKT7-53 does not interact with the protein product of the empty pGADT7 vector (empty).