Table 6.
The effect of BetA on the mRNA expression levels of cell cycle genes in breast carcinoma MDA-MB-231 and MDA-MB-468 cell lines and their co-culture with breast stromal cellsa.
| BetA concentration (µg/mL) | p21 | P27 | CDK2 | CDK6 | Cyclin Dl | |
|---|---|---|---|---|---|---|
| MDA-MB-231 | 1 | 0.26 | 0.10 | 0.32 | 0.12 | 0.36 |
| 10 | 0.42 | 0.14 | 0.42 | 0.12 | 0.40 | |
| 20 | 0.74 | 0.29 | 0.69 | 0.25 | 1.12 | |
| 5b | 0.19 | 0.32 | 2.22 | 1.07 | 0.23 | |
| MDA-MB-468 | 5 | 1.67 | 7.60 | 2.32 | 3.54 | 1.85 |
| 10 | 2.49 | 4.68 | 0.73 | 5.16 | 2.59 | |
| 20 | 3.45 | 10.21 | 4.13 | 5.06 | 2.99 | |
| 5b | 0.55 | 2.44 | 1.21 | 3.15 | 1.91 | |
| 10b | 6.27 | 6.70 | 1.07 | 2.80 | 0.95 |
a
The values represent fold change in expression of the target gene relative to the internal control gene (β-actin) and untreated control cells.
b
Breast carcinoma cell line co-cultured with breast stromal cells.