Figure 3.

Role of Bad in the combination of mitomycin C and rapamycin-induced apoptosis. LS174T (a) and LS180 (b) cells were treated with mitomycin C (5 μg/ml) and/or rapamycin (2.5–10 μg/ml) for 24 h, p-Bad/Bad were detected by immunoblotting. Actin was used to confirm the equal amount of proteins loaded in each lane. (c) LS174T cells were treated with mitomycin C (5 μg/ml) and/or rapamycin (10 μg/ml) for 24 h. After treatment, cell lysates were immunoprecipitated with anti-Bad antibody or IgG and immunoblotted with anti-Bcl-xL antibody. The presence of Bad and Bcl-xL in the lysates was examined. (d) After treatment, cell lysates were immunoprecipitated with anti-Bak antibody or IgG and immunoblotted with anti-Bcl-xL antibody. The presence of Bak and Bcl-xL in the lysates was examined