Figure 6.

Effect of mitomycin C and rapamycin on the growth of LS174T intraperitoneal tumors. (a) Nude mice were intraperitoneally inoculated with 5 × 10⁵ LS174T-luc cells/mouse on day 0. Four days after tumor inoculation, all the tumor-bearing mice were treated with either PBS alone (sham) or rapamycin alone at a dose of (0.25 mg/kg body weight) or treated with mitomycin C (1.5 mg/kg body weight) alone or treated with combination of mitomycin C and rapamycin every other day from day 4 to day 28. Mice were imaged using the IVIS Imaging System Series 200. (b) Line graph illustrating the luciferase activity (photons/second) in LS174T tumor-bearing mice treated with PBS alone, rapamycin alone or mitomycin C alone or combination of rapamycin with mitomycin C from week 1 to week 4. ** represents a statistically significant difference compared with the control group. ^## represents a statistically significant difference compared with the single treatment group. (c) Tumor tissues were harvested at day 28 and subjected to H&E staining, immunohistochemistry staining with anti-CEA and Ki67 primary antibodies and TUNEL assay. Cell nuclei were stained with DAPI. Representative images are shown (magnification × 400)