Figure 4.

The organization of actin stress fibers was not affected by WNT3a or sFRP1. Primary GTM cells were treated with WNT3a (100 or 500 ng/mL), sFRP1 (2 or 10 μg/mL), Y27632 (10 μM), or LPA (20 μM) for 24 hours. Actin stress fibers were stained with phalloidin-Alexa-594. Neither WNT3a nor sFRP1 induced significant changes in actin stress fibers. For comparison, the ROCK inhibitor Y27632 disrupted actin stress fiber formation, while the Rho activator LPA enhanced actin stress fiber formation.