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A flow chart for Basic Protocol 1 is shown. Basic Protocol 1, step 14, separates crude ER (supernatant) from crude mitochondria (pellet). Subsequent steps are grouped by the organelle which is to be purified for clarity and to provide a sense of continuity. To streamline the timing of the procedure and to reduce protein degradation; however, ER and mitochondrial purification steps should be carried out simultaneously.
