Table 3.27.2.
Cellular Markers for Verification and Assessment of ER, Mitochondria, and MAM Subcelluar Fractions
| Subcellular compartment | Marker | Antibody | Vendor | Reference |
|---|---|---|---|---|
| ER (microsomes) | DPM1 | I-20 | Santa Cruz Biotechnology cat. no. sc-15836 | Maeda et al., 1998 |
| Calreticulin | Affinity Bioreagents cat. no. PA3-900 | Johnson et al., 2001; Gelebart et al., 2005 | ||
| Mitochondria | SDH | MitoSciences | Lynes et al., 2012 | |
| ATPase-α | Invitrogen no.459240 | Guardia-Laguarta et al., 2014 | ||
| COXII | K-20 | Santa Cruz Biotechnology cat. no. sc-23984 | Scheffler, 2001 | |
| Grp75 | Stressgen cat. no. SPS-825 | Manning-Krieg et al., 1991 | ||
| MAM | mEGFP-huPSS-1 | B-2 (anti-GFP) | Santa Cruz Biotechnology cat. no. sc-9996 | Bozidis et al., 2008 |
| huPSS-1 | Y-19 | Santa Cruz Biotechnology cat. no. sc-51410 | Morita et al., 2012 | |
| FACL4 | Abgent cat. no. AP2536b | Simmen et al., 2005 | ||
| Sig-1R | Santa Cruz Biotechnology | Williamson et al., 2011 | ||
| Erlin-2 | Cell Signaling Technology cat. No. 2959 | Guardia-Laguarta et al., 2014 | ||
| Mfn2 | Abcam | Zhang et al., 2011 | ||
| ACAT1 | Affinity Bioreagents | Myhill et al., 2008 | ||
| TMX1 | Sigma | Lynes et al., 2012 | ||
| TMX2 | Lifespan Biosciences | Lynes et al., 2012 | ||
| α-synuclein | Cell Signaling Technology cat. No. 2628 | Guardia-Laguarta et al., 2014 | ||
| p66Shc | Abcam | Wieckowski et al., 2009 |