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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Nat Protoc. 2015 Aug 20;10(9):1407–1422. doi: 10.1038/nprot.2015.092

Table 3.

Configuration of LSR II.

Detector Long-pass filter Band-pass filter Fluorochrome
Blue laser (488 nm)
A 685LP 695/40 PerCP-Cy5.5
B 505LP 530/30 FITC, CFSE
C 488/10 SSC
Yellow/green laser (561 nm)
A 750LP 780/60 PE-Cy7
D 600LP 610/20 PE Texas Red
E 582/15 PE
Red laser (633 nm)
A 735LP 780/60 APC-Cy7
B 690LP 710/50 Alexa Fluor 700
C 660/20 APC
Violet laser (405 nm)
A 635LP 655/20 Qdot 655, BV650
B 595LP 605/20 BV605
C 505LP 525/50 V500, BV510
D 450/50 BV421
UV laser (355 nm)
A 735LP 780/60 Qdot 800
B 410LP 450/50 Live/Dead blue
C 379/28 BUV395