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. Author manuscript; available in PMC: 2016 Sep 10.
Published in final edited form as: Mol Biochem Parasitol. 2015 Sep 10;202(1):29–37. doi: 10.1016/j.molbiopara.2015.09.001

Figure 2. SmGAR forms a constitutively active receptor that responds selectively to acetylcholine.

Figure 2

(A) Yeast cells were transformed with SmGAR expression plasmid (black bars) or empty plasmid (mock, red bars). Cells were then assayed for receptor activity in the presence of test substances each at 10−4 M or vehicle (water). The results are from a single clone of SmGAR but are representative of 3 separate clones. *** Significantly different from water-treated control at P< 0.0001. ACh (B) and carbachol (C) activate SmGAR-expressing cells (black triangles) in a concentration-dependent manner. No effect was observed in the mock control at any of the concentrations tested (red squares).