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. Author manuscript; available in PMC: 2016 Sep 24.
Published in final edited form as: Cell. 2015 Sep 17;163(1):108–122. doi: 10.1016/j.cell.2015.08.010

Figure 5. BuGZ coacervation promotes MT polymerization by concentrating tubulin.

Figure 5

A. Tubulin was concentrated in droplets formed by YFP-xBuGZ, but not by YFP-xBuGZΔN. Scale bar, 20 μm.

B. Anti-His-tag antibody pulled down indicated xBuGZ and tubulin at 4°C.

C. Illustration of the spin down assay.

D. Higher concentrations of YFP-xBuGZ and tubulin are found in droplets (Y-axis) than in initial solution concentrations (X-axis).

E–F. Compared to initial solution concentrations (X-axis), YFP-xBuGZΔN (E) coacervation only concentrated itself, but not tubulin, in droplets (Y-axis), whereas YFP-xBuGZ13S (F) did not coacervate or concentrate itself or tubulin (Y-axis).

G–H. Representative fields of MTs polymerized. MTs were counted in 20 random microscopic fields using a 63× objective. Scale bar, 10 μm.

Error bars, SEM. Student’s t-test: ns, not significant, *p<0.05, **p<0.01, ***p<0.001, three independent experiments.