Fig 5. CCR2 is required for host survival and the large translocation-dependent ET cell response.
Age and sex-matched C57BL/6 (filled symbols) or C57BL/6 Ccr2-/- mice (open symbols) were left uninfected or infected IV with 1000 CFU of mE (circles) or 5X105 CFU of ΔBmE (squares). Mouse survival (A) and weight (B) following infection with mE were monitored for 14 days. Data shown in (A) and (B) are from 1 experiment with 6 mice in each condition. Difference in survival was significant as determined with Log rank test. The differences in weight between the two groups of mice at different days were determined with two-way ANOVA followed by Bonferroni posttest. *, P<0.05; ***, P<0.001. (C) At different dpi as indicated with mE or 7 dpi with ΔBmE, spleen colonization levels were determined by CFU assay. (D) Representative histographs of Ly6C and CD11b signals on splenocytes from uninfected (UI) or mE-infected C57BL/6 or Ccr2-/- mice at 7 dpi, and the CD11b+Ly6Chi cells are indicated with an oval gate (percentage of total splenocytes within gate is shown). (E) The number of ET cells at 7 dpi was determined by flow cytometry following tetramer staining. Data shown in (C) and (E) are the summary of several independent experiments with at least two experiments at each time point. P values were determined with Mann-Whitney test as indicated in (C) or (E).