Table 1. Y. pseudotuberculosis strains used in the study.

Strain name	Relevant characteristic(s)	Reference or source
32777	Serogroup O:1, pYV+	[44]
mE	yopE R144A, catalytically inactive allele of yopE	[44]
ΔB	yopB40, stop codon and frame shift in yopB	[44]
ΔBmE	yopB40, yopE R144A	This study
ΔGAP	yopE ΔGAP, deletion of codons corresponding to GAP domain	This study
ΔpYV	Cured of the virulence plasmid pYV	[45]
ΔYscF	ΔyscF, deletion of entire open reading frame	This study
YopE-Bla	Integration of plasmid into yopE in pYV generates translational fusion of chaperone-binding domain of YopE and TEM1 β-lactamase	This study
ΔB YopE-Bla	yopB40, integration of plasmid into yopE in pYV generates translational fusion of chaperone-binding domain of YopE and TEM1 β-lactamase	This study
YopEΔN15	yopE Δ2–15, deletion of codons corresponding to signal sequence residues 2–15	This study
ΔYopE	ΔyopE, deletion of entire ORF	This study