Extended Data Table 2.
Data summary table for the single vesicle-vesicle fusion experiments with Syt1 and SNAP-25 mutants
| No. of spontaneous fusion events | No. of Ca2+ triggered fusion events | Total no. of analyzed events (no. of associated vesicle pairs) | No. of independent experiments (N) | |
|---|---|---|---|---|
| Syt1 WT | 90 | 167 | 3764 | 5 |
| 100 | 262 | 4632 | 6 | |
| 49 | 152 | 3035 | 5 | |
| 64 | 173 | 3564 | 4 | |
| 159 | 256 | 6503 | 9 | |
| Syt1 mutants | ||||
| E295A, Y338W | 113 | 246 | 5872 | 12 |
| R398Q, R399Q | 166 | 217 | 6724 | 7 |
| R281A, R398A, R399A | 133 | 271 | 5134 | 7 |
| Syt1 quintuple | 49 | 143 | 5241 | 7 |
| SNAP-25 mutants | ||||
| K40A, D166A | 61 | 139 | 2549 | 7 |
| D51A, E52A, E55A | 81 | 150 | 2717 | 7 |
| SNAP-25 quintuple | 105 | 261 | 6741 | 9 |
| Control | ||||
| +ATP | 59 | 129 | 2830 | 5 |
| -SNAP-25 | 24 | 36 | 3040 | 5 |
| -Syt1 | 57 | 58 | 2379 | 5 |
| Mock | - | 25 | 1947 | 5 |