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. 2015 Oct 15;10(10):e0140591. doi: 10.1371/journal.pone.0140591

Fig 3. Purification of the 6xHis:TaCRT1 protein (A) and Scatchard plot of calcium binding to the affinity-purified TaCRT1 protein (B).

Fig 3

The 6xHis and 6xHis:TaCRT1 proteins isolated from non-induced cells (lanes 1 and 3) and IPTG-induced cells (lanes 2 and 4) and purified TaCRT1 proteins obtained after thrombin cleavage (lane 5) were resolved on an SDS-PAGE gel and stained with Coomassie Blue. Lanes 1–4 contain 2 μg of protein each.