Table 2. Effect of seed priming with PEG and soil moisture stress on leaf, root, and stem RWC in sorghum seedlings.a .
RWC (%) | |||||||
---|---|---|---|---|---|---|---|
12 DAP | 24 DAP | ||||||
ST b | SMT | Leaf | Root | Stem | Leaf | Root | Stem |
Unprimed | Unstressed control | 87.6 | 84.6 | 81.1 | 86.6 | 82.3 | 79.3 |
Drought | 73.1 | 69.1 | 68.3 | 75.3 | 73.3 | 72.3 | |
EM | 78.1 | 75.1 | 74.6 | 79.6 | 77.3 | 73.3 | |
Drought -EM | 81.3 | 77.3 | 76.6 | 82.3 | 79.1 | 77.6 | |
EM-drought | 80.2 | 76.2 | 75.5 | 80.6 | 78.1 | 75.5 | |
Primed | Unstressed control | 89.2 | 87.2 | 83.2 | 88.5 | 84.2 | 82.1 |
Drought | 81.6 | 79.6 | 79.5 | 83.3 | 77.3 | 76.2 | |
EM | 84.2 | 81.2 | 78.8 | 85.9 | 82.2 | 79.2 | |
Drought -EM | 85.1 | 80.1 | 80.3 | 86.2 | 82.3 | 80.2 | |
EM-drought | 86.2 | 82.2 | 79.4 | 86.0 | 80.5 | 80.3 | |
LSD0.05 | 4.7 | 4.4 | 5.0 | 4.0 | 4.5 | 3.7 | |
ST F-value | 37.3* | 43.1* | 32.7* | 35.1* | 16.2* | 38.5* | |
SMT F-value | 16.0* | 18.0* | 9.2* | 9.4* | 9.8* | 11.7* | |
ST× SMT F-value | 1.6 ns | 2.6 ns | 3.2* | 1.1 ns | 0.4 ns | 0.9 ns |
Numbers represent F-values at 0.05 probability level
*, ns Significant and non-significant at 0.05 probability level
aSeeds were primed with 20% (w/v) PEG 8000 at 18°C for 24 h. The unprimed and primed sorghum seeds were germinated under unstressed soil moisture environment (Unstressed control), drought, EM, 8 d drought stress followed by EM, and 8 d EM followed by drought stress, respectively.
bAbbreviations: DAP, days after planting; EM, excessive soil moisture; LSD, least significant difference; PEG, polyethylene glycol; RWC, relative water content; ST, seed treatment; SMT, soil moisture treatment