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. 2015 Oct 15;10(10):e0140620. doi: 10.1371/journal.pone.0140620

Table 2. Effect of seed priming with PEG and soil moisture stress on leaf, root, and stem RWC in sorghum seedlings.a .

RWC (%)
12 DAP 24 DAP
ST b SMT Leaf Root Stem Leaf Root Stem
Unprimed Unstressed control 87.6 84.6 81.1 86.6 82.3 79.3
Drought 73.1 69.1 68.3 75.3 73.3 72.3
EM 78.1 75.1 74.6 79.6 77.3 73.3
Drought -EM 81.3 77.3 76.6 82.3 79.1 77.6
EM-drought 80.2 76.2 75.5 80.6 78.1 75.5
Primed Unstressed control 89.2 87.2 83.2 88.5 84.2 82.1
Drought 81.6 79.6 79.5 83.3 77.3 76.2
EM 84.2 81.2 78.8 85.9 82.2 79.2
Drought -EM 85.1 80.1 80.3 86.2 82.3 80.2
EM-drought 86.2 82.2 79.4 86.0 80.5 80.3
LSD0.05 4.7 4.4 5.0 4.0 4.5 3.7
ST F-value 37.3* 43.1* 32.7* 35.1* 16.2* 38.5*
SMT F-value 16.0* 18.0* 9.2* 9.4* 9.8* 11.7*
ST× SMT F-value 1.6 ns 2.6 ns 3.2* 1.1 ns 0.4 ns 0.9 ns

Numbers represent F-values at 0.05 probability level

*, ns Significant and non-significant at 0.05 probability level

aSeeds were primed with 20% (w/v) PEG 8000 at 18°C for 24 h. The unprimed and primed sorghum seeds were germinated under unstressed soil moisture environment (Unstressed control), drought, EM, 8 d drought stress followed by EM, and 8 d EM followed by drought stress, respectively.

bAbbreviations: DAP, days after planting; EM, excessive soil moisture; LSD, least significant difference; PEG, polyethylene glycol; RWC, relative water content; ST, seed treatment; SMT, soil moisture treatment