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. 2015 Oct 15;10(10):e0140824. doi: 10.1371/journal.pone.0140824

Fig 2. DENV-3 entry into Vero cells is dependent on dynamin.

Fig 2

(A) Cells were treated with dynasore and infected with DENV-3. After 1h of internalization in presence of the drug, monolayers were treated with proteinase K and the cell pellets were plated onto Vero cells to determine internalized virus by an infectious centre assay. (B) Cells treated with 150 μM dynasore or untreated (control) were infected with DENV-3. At 48 h p.i., immunofluorescence staining was carried out using mouse anti-E glycoprotein antibody. (C) Cells transiently transfected with GFP-Dyn II wt or GFP-Dyn II K44A were infected with DENV-3. After 24 h, cells were fixed and viral antigen expression was visualized by immunofluorescence staining using mouse anti-E glycoprotein antibody and TRITC-labelled anti-mouse IgG. (D) For quantification of samples shown in C, 250 transfected cells with similar levels of GFP expression were screened and cells positive for viral antigen were scored. In (A) and (D) results are expressed as the mean of three independent experiments ± SD. Asterisks indicate statistical significance (** p < 0.01, *** p < 0.001).