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. 2015 Oct 15;10(10):e0140824. doi: 10.1371/journal.pone.0140824

Fig 5. Transport of DENV-3 particles to early and late endosomes.

Fig 5

Cells transiently transfected with the GFP-tagged versions of Rab5 wt and S34N (A, B) or Rab7 wt and T22N (C, D) were infected with DENV-3. After 24 h cells were fixed and viral antigen expression was visualized by immunofluorescence staining using mouse anti-E glycoprotein antibody and TRITC-labelled anti-mouse IgG. (B)(D)For quantification of samples, 250 transfected cells with similar levels of GFP expression were screened and cells positive for viral antigen were scored. Results are expressed as the mean of three independent experiments ± SD. Asterisks indicate statistical significance (** p < 0.01).