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. 2015 Oct 15;10(10):e0139977. doi: 10.1371/journal.pone.0139977

Fig 3. STC1 suppressed HCC tumor growth in vitro.

Fig 3

Spheroid formation and cell migration assay were performed in both stably STC1-overexpressed Hep3B (A-B) and 97L cell lines (C-D). The sizes of spheroids were measured at 10 days of the incubation. At least 3 random fields representing 200 spheroids were counted under 200x magnification. (A) In Hep3B cells, the representative pictures of spheroids were shown (top). (C) In 97L cells, overexpression of STC1 was confirmed by probing with V5 antibody (right). Significant reductions in sizes of the spheroids in Hep3B/STC1 and 97L/STC1 were noted. * p<0.05, **p<0.01, compared with empty vector transduced cells. Bars = 50 μm. The activities of cell migration in transwells were induced in (B) Hep3B cells and (D) 97L cells by the treatment with 50ng/ml of IL6 or IL8 for 24h. At least 5 random fields were counted under 100x magnification. The pro-migratory effects of IL6 or IL8 on Hep3B/STC1 and 97L/STC1 were significantly suppressed by STC1-overexpression. * p<0.05, ** p<0.001, compared with the treatments in the respective pLenti-transduced cells; # p<0.00001, compared with the same treatment group.