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. 2015 Oct 15;10(10):e0140320. doi: 10.1371/journal.pone.0140320

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© 2015 Kay et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — HA-hMC4R was transiently transfected into HEK293 cells stably expressing DsRed-ER, a marker of the ER (A, C, E) or GalTase-mCherry, a marker of the Golgi apparatus (B, D, F). Z-stacks for HA-hMC4R with each marker were acquired using confocal microscopy and representative single slices through the approximate centre of each cell are shown. HEK293 cells were counterstained with Hoechst or DAPI to determine signal orientation. The white arrows indicate HA-hMC4R signal in close proximity to the cell membrane. Co-localisation analysis for the data represented in this figure is shown in S2A Fig Co-localisation of HA-hMC4R and DsRed-ER or GalTase-mCherry is observed as yellow spots. Scale bars = 10μm.