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. 2015 Oct 15;10(10):e0140320. doi: 10.1371/journal.pone.0140320

Fig 5. hMC4R-eGFP was predominantly observed in stable clusters of vesicles associated with a marker of the ER when expressed alone in live HEK293 cells.

Fig 5

hMC4R-eGFP was transiently transfected into HEK293 cells stably expressing DsRed-ER (A, B) or GalTase-mCherry (C, D) and live confocal microscopy was performed with XY scans acquired every 10 seconds. hMC4R-eGFP dynamics at the cell membrane of dsRed-ER stable cells are indicated by the white arrows in the middle set of panels of sections B and D. Stable clusters containing hMC4R-eGFP and DsRed-ER or dynamic vesicles containing hMC4R-eGFP and GalTase-mCherry are indicated by the white arrows in the bottom panels of sections B and D, respectively. Scale bars = 10μm and 2μm for insets (B, D).