Fig. 2.—

Hydrogenosomal proteins are targeted to yeast mitochondria with and without N-terminal leaders. (A–D) All four hydrogenosomal proteins of Trichomonas vaginalis analyzed (TvSCSα1, succinyl coenzyme A synthetase; TvFdx, ferredoxin; TvME, malic enzyme; TvISCA1; iron–sulfur assembly protein 1) are targeted to the mitochondria of yeast, even in the absence of their NTSs. Δ indicates the positions of the N-terminal amino acids (i.e., the hNTS) that were deleted. (E) Trichomonas actin was used as a control next to the transfection of the empty vector that expresses GFP alone (supplementary fig. S2, Supplementary Material online). Scale indicates 5 µm. On the right, the multiplex western blots of isolated mitochondria probed with anti-GFP antibody (green) and the mitochondrial marker protein anti-COXIV (red). TL, total lysate; Cy, cytosol; Mt, mitochondria. Numbers to the left indicate the approximate molecular weights of the constructs in kilo Daltons (kDa).