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. 2015 Sep 2;7(9):2716–2726. doi: 10.1093/gbe/evv175

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© The Author(s) 2015. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Fig. 4.— — Cytosolic proteins do not colocalize with the organelles. (A) Expression of TvActin (Trichomonas actin) and the empty vector expressing the eGFP tag alone demonstrates that they do not localize to the mitochondria stained with MitoTracker® Red. (B) Three cytosolic proteins from yeast (ScGAPDH, glyceraldehyde-3-phosphate dehydrogenase; ScActin, actin; ScRab5, rab family GTPase) were expressed using the same expression vector and yet do not colocalize with the hydrogenosomal marker TvSCSα1.