Table II.
Primers Used to Sequence Across Breakpoints and Products Generated
| Cells/Deletion | primer closest to5’ end of deletion | primer closest to3’ end of deletion | expected maximum size of PCR product | actual observed size of PCR product |
|---|---|---|---|---|
| TK6 | TTC19int7eF | PIGLex3R | 2469 bp | ~2000 bp |
| A | TTC19int7eF | PIGLex3R | 2469 bp | ~2000 bp |
| B | NCOR1 int15R | PIGLint2kR | 1526 bp | no product |
| C | NCOR1int1hR | PIGL int2bR | 5798 bp | ~4000 bp |
| D | NCOR1 int15dR | PIGL int2bR | 3826 bp | ~1500 bp |
| E | NCOR1 int14R | PIGLex3R | 3720 bp | no product |
| F | NCOR1 int19R | PIGLex3R | 5512 bp | no product |
| G | NCOR1int18bR | PIGL int2bR | 5366 bp | ~3500 bp |
*
based on distance of primers from breakpoint