Table 1. Axon Tracing Task Results.

Lengths of tracings of designated axons as visualized first by YFP, then by BDA. In three cases individuals’ tracing ended on incorrect YFP-labeled axons (*), and this occurred in one case based on BDA-labeling, from a novice axon tracer. Overall tracings based on visualization by YFP were significantly shorter than tracings by visualization by BDA (p = 0.0402, two-tailed paired t-test), as were tracings in which incorrectly traced axons were excluded (p = 0.0494, two-tailed paired t-test).

	YFP tracing			BDA tracing
	Axon 1	Axon 2	Axon 3	Axon 1	Axon 2	Axon 3
Tracer 1: experienced	15.9*	96.6*	58.0	172.6	122.9	184.5
Tracer 2: novice	12.2	8.5*	32.4	170.5	157.3	214.7*
Tracer 2: novice	26.6	61.4	59.4	171.9	124.3	202.2
Tracer 4: experienced	32.1	78.7	59.6	159.1	123.9	184.5