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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Bioorg Med Chem Lett. 2015 May 27;25(21):4910–4917. doi: 10.1016/j.bmcl.2015.05.046

Figure 4. Binding isotherms generated using affinity-purified antibody.

Figure 4

Antibody from NOD4 serum was purified over a column displaying 1, and binding of the IgG antibody to 1 was measured quantitatively using a flow cytometry-based assay. Beads were incubated with a 2-fold serial dilution of serum, starting from 270 µg mL−1. The eluted antibody fraction exhibited saturating binding to 1 with a Kd of 40 nM.