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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: J Pathol. 2015 Jul 14;237(3):285–295. doi: 10.1002/path.4574

Figure 5.

Figure 5

CD47 deficiency decreases the production of Tfh cells. Splenocytes from WT and CD47−/− mice were stimulated with LPS in the presence or absence of IL-4 for 4 days. A) Splenic B cell proliferation was assessed using BrdU assay in vitro after LPS stimulation. B) IgG, IgM and IgA in the culture medium of splenocytes stimulated with LPS were detected by ELISA (n=5). C) IgG1 and IgG3 antibodies were measured by ELISA (n=5). D–F) Male WT or CD47−/− mice (n = 4 to 6) were immunized i.p. with peptide in CFA/IFA, followed by splenocyte collection at day 7 after the fourth immunization. D) The frequency of follicular T cells in spleen and lymph nodes stained with APC-CD4 mAb and PE-CXCR5 mAb. E–F) The number of CD4+ CXCR5+ T follicular cells in spleen (E) and lymph nodes (F). **, P<0.01.