Figure 6.

Blocking of CD47 by anti-CD47 antibody impaired IgG production in peptide/CFA-immunized WT mice but aggravated glomerulonephritis in Fas^lpr mice. In each experiment, six male WT or CD47^−/− mice were treated with IgG control or anti-CD47 antibodies followed by peptide in CFA/IFA immunization. A) Serum IgG levels measured by ELISA every 7 days after immunization. B) Impairment of GC formation and reaction in immunized mice treated with anti-CD47 antibody on day 7. The sections of mouse spleen white pulp were stained with anti-IgM mAbs (red) and peanut agglutinin (PNA; green) to show GC architecture. C) Kinetics of total serum IgG levels in mice treated with IgG control or anti-CD47 antibodies after 8 weeks (wk). D) Proteinuria levels assayed by BCA methods. The results in panel A–D were presented as mean ± SE from five independent experiments. **, P<0.01.