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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Proteomics. 2015 May 28;15(20):3424–3438. doi: 10.1002/pmic.201400571

Figure 1. Human gut metaproteomics workflow.

Figure 1

Collected fecal samples can be processed by direct or indirect protein extraction methods. For the direct method, the entire fecal material is prepared via chemical/mechanical cell lysis for protein extraction, while indirect method first enriches microbial cells via differential centrifugation or density gradient prior to cellular lysis. The proteolytic peptide mixtures are analyzed via two-dimensional (2D)–LC-MS/MS or by 2D PAGE (polyacrylamide gel electrophoresis) approaches.