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. 2015 Jun 4;21(1):505–514. doi: 10.2119/molmed.2015.00073

Figure 3.

Figure 3

The effect of AGA(C8R)-HNG17 on intracellular ATP production. (A) Intra-cellular ATP levels of PC-12 cells treated with different concentrations of AGA(C8R)-HNG17 under necrotic conditions induced by chemohypoxia with KCN or by STS/oligomycin-A. ATP levels were detected by measuring bioluminescence in cells extracts via the luciferin–luciferase method. *P ≤ 0.05 (t test) compared with treatment with KCN with vehicle; n = 3; **P ≤ 0.05 (t test) compared with treatment with oligomycin-A and STS with vehicle; n = 3. (B) The effect of different concentrations of AGA(C8R)-HNG17 on the activity of ATP synthase (relative to vehicle) isolated from rat liver mitochondria, measured by following the decrease in NADH absorbance at 340 nm. The average rate of decrease in NADH absorbance of the control was 0.97 ± 0.06 mAU/min. *P ≤ 0.005 (t test) compared with vehicle, n = 3.