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A depiction of how the Gelfree mass fractionation helps mitigate the confounding effects of ambiguous peptides. The left panel illustrates how discerning between proteins is not possible given an ambiguous peptide in a sample containing both proteins. The right panel illustrates how separating the proteins by mass using Gelfree prior to analysis helps eliminate the ambiguity by ensuring that the sampled peptide can only be from a protein from the respective mass range
