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Fluorescence assay of the effect of umbelliferone and trolox on Fe-mediated ROS production monitored by DCF emission. All solutions were prepared in PBS (pH 7.4) with 10 µM FeCl3, 10 µM NTA, 1 mM H2O2 and 1 mM ascorbate. Condition labeled “NTA” contains only Fe, NTA, H2O2, and ascorbate.
