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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Bioorg Med Chem Lett. 2015 Jul 17;25(21):4770–4773. doi: 10.1016/j.bmcl.2015.07.033

Figure 3.

Figure 3

4 selectively inhibits the auto-ADP-ribosylation activity of full-length LG-PARP10. HEK 293T cells overexpressing GFP-WT-PARP10, GFP-LG-PARP10, or GFP-GW-PARP10 were harvested 24 h after transfection, and GFP-tagged PARP10 proteins were immunoprecipitated using an anti-GFP antibody. Auto-ADP-ribosylation assays were performed on immunoprecipitates using 6-a-NAD+. After click conjugation with biotin-azide proteins were resolved by SDS/PAGE and detected by Western blot with either Streptavidin-HRP or an antibody against GFP.