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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Bioorg Med Chem Lett. 2015 Aug 10;25(21):4733–4739. doi: 10.1016/j.bmcl.2015.08.021

Figure 2.

Figure 2

Concentration-response curves for endogenous agonist 2 (blue), short-chain conjugate 10 (red) and long-chain conjugate 11 (black) for inhibition of forskolin-stimulated cAMP formation in P2Y14R-expressing CHO cells, as described.14 Incubations were at 37 °C for 15 min and were terminated by aspiration of medium and addition of 500 μL of ice-cold 5% trichloroacetic acid. All assays with 6, 22, and 30 also contained 30 μM forskolin and 200 μM IBMX, 3-isobutyl-1-methylxanthine, a phosphodiesterase inhibitor. Data points for IBMX alone (indicated by IBMX in the legend) and forskolin + IBMX (indicated by forskolin in the legend) also are shown.