Figure 6. TIM-1 trafficking following recognition of apoptotic cells.
Confocal images of 300.19 preB cells transfected with mTIM-1-YFP or a MILIBS mutant (both in green) before (left) or after addition of 7AAD-labelled apoptotic 300.19 cells (red; center and right). Conjugates formed 4 h after addition of apoptotic cells are shown. The plot shows the percentage of TIM-1 protein that accumulated at the contacts of transfected and apoptotic cells. Bar = (5 μm). Student’s t-test; *** P <0.001. Representative images from 3 experiments are shown.