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. Author manuscript; available in PMC: 2015 Oct 16.
Published in final edited form as: Cell Rep. 2015 Oct 1;13(2):260–266. doi: 10.1016/j.celrep.2015.08.086

Figure 3. The preE loop of pre-let-7c-2 precludes LIN28A binding and regulation.

Figure 3

(A) Representation of different wild type and mutant preE sequences of let-7 constructs used in EMSA and transfection experiments. Mutations are underlined, preE stem is in blue and GGAG motif is in red. (B) EMSA showing specific binding of LIN28A to individual let-7 wild type and mutant pre-miRNA depicted in (A). (C) q.RT-PCR analyses of let-7a, let-7c and let-7g expression in Hela cells upon transfection of empty vectors (mock), let-7 pri-miRNA only or let-7 pri-miRNA and FLAG-LIN28A as indicated. Results are the average of two independent experiments. Error bars represent SD.