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. Author manuscript; available in PMC: 2015 Oct 16.
Published in final edited form as: Cell Rep. 2015 Oct 1;13(2):326–336. doi: 10.1016/j.celrep.2015.08.082

Figure 5. Pdx1 and Sox9 cooperatively silence genes encoding intestinal cell fate regulators.

Figure 5

(A) Illustration of the experimental strategy for gene expression microarray analysis. The mRNA profiles of embryonic day (E) 12.5 pancreata (n=12 per genotype) from 1.) Pdx1+/− versus Pdx1+/−;Sox9+/Δgut and 2.) Sox9+/Δgut versus Pdx1+/−;Sox9+/Δgut littermates were compared. (B) 3,337 and 4,486 genes were differentially-expressed between 1.) and 2.), respectively. A total of 1,817 genes were common to both sets of significantly-regulated genes (FDR<0.05) with the same sign of change (i.e. up-regulated or down-regulated). (C) Pdx1- and Sox9-co-regulated genes were identified by cross-comparing mRNA profiles of E12.5 pancreata (n=12 per genotype) from 1.) Pdx1+/− versus Pdx1+/−;Sox9+/Δgut and 2.) Sox9+/Δgut versus Pdx1+/−;Sox9+/Δgut littermates. A total of 1,817 genes (denoted by red pixels) were common to both sets of significantly-regulated genes (FDR<0.05) with the same sign of change. (D) Gene ontology analysis of the 1,817 Pdx1- and Sox9-co-regulated genes. (E) The top twenty Pdx1- and Sox9-co-repressed genes with the highest fold-change.