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. Author manuscript; available in PMC: 2016 Jan 2.
Published in final edited form as: Protein Expr Purif. 2014 Oct 13;105:14–22. doi: 10.1016/j.pep.2014.09.020

Fig. 3.

Fig. 3

Reverse-phase HPLC chromatogram profiles for P71T (A) and M180 (B) illustrating the improvement in yields due to dialysis in 2% acetic acid and cell lysis with 6 M guanidine hydrochloride, respectively. In both chromatograms the major band eluting at ~36 min contains the amelogenin protein and corresponds to ~68% Buffer B (70% aq. CH3CN in 0.1%). (A) Soluble fraction of the cell lysis material from P71T, prepared by heating in 2% acetic acid, following centrifugation without (red) and with (black) extensive dialysis in 1–2% acetic acid. (B) Soluble fraction of the cell lysis material from M180, prepared by heating in equal volumes of 2% acetic acid (black) or 6 M guanidine hydrochloride (purple). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)